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Using the PCr and Gly concentrations determined in this study, the modeling of depletion of the Mb-O 2 , PCr and Gly stores during dives was based on the following assumptions: (1) no muscle blood flow occurred during the dives (Meir et al. 2011); (2) locomotory muscle metabolic rate was constant after the first minute at a rate of 12.4 ml O 2 kg −1 muscle min −1 (Williams et al. 2011); (3) in addition to a muscle metabolic rate of 12.4 ml O 2 kg −1 muscle min −1 during the first min, PCr declined by 30% during the first minute based on the work-rest transition phase requirements demonstrated in exercise physiology studies (Barstow et al. 1995); (4) Mb-O 2 depletion continued to provide the required ATP until 6 min into a dive; during that time, aerobic metabolism of glucose from the Gly store provided 67% of the metabolic substrate; it is assumed that intracellular fat provided the remainder (Hochachka and Somero 1984); (5) once Mb-O 2 declined to 15–20% saturation, PCr and Gly depletion, at a 1:2 ratio (Kemp et al. This assumption was based on a previously shown rise in lactate prior to complete Mb-O 2 depletion (Scholander et al.

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