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Muscle biopsies were obtained from ten penguins (mass: 23.6 ± 1.9 kg; range: 21–26 kg) under isoflurane anesthesia (Kooyman et al. 1992) using a Bergstrom muscle biopsy needle (Bergstrom 1962). All muscle biopsies were taken from the pectoralis-supracoracoideus complex, 3–4 cm lateral to midline at the level of the axilla. Biopsy samples for Mb, PCr, creatine (Cr), Gly, lactate and water content analysis were obtained from a muscle depth of 6 – 8 cm and frozen immediately in liquid nitrogen (N 2 ).

Samples for water content analysis were weighed prior to being frozen.

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